Simultaneous quantification of polysorbate 20 and poloxamer 188 in biopharmaceutical formulations using evaporative light scattering detection
نویسندگان
چکیده
منابع مشابه
Evaporative light scattering detection of pyrrolizidine alkaloids.
A reverse-phase high-performance liquid chromatography method utilizing evaporative light scattering detection (ELSD) has been developed for the simultaneous detection of hepatotoxic pyrrolizidine alkaloids with and without chromophores, namely, riddelliine, riddelliine N-oxide, senecionine, senecionine N-oxide, seneciphylline, retrorsine, integerrimine, lasiocarpine and heliotrine. Pyrrolizidi...
متن کاملQuantification of phosphatidic acid and lysophosphatidic acid by HPLC with evaporative light-scattering detection.
Phosphatidic acid (PA) and lysophosphatidic acid (LPA) are lipids that regulate cellular processes. PA stimulates kinases and may play a role in exocytosis and membrane fusion. LPA can induce cell proliferation, platelet aggregation, and microfilament formation. Due to the growing interest in these lipids, rapid purification and quantification of these lipids is desirable. We now describe a met...
متن کاملDetectors: Evaporative Light Scattering
Rcation of short-lived free radicals by an electron spin resonance trapping technique. Journal of the American Chemical Society 90: 5909}5910. Kroll C, Osterloh K, MaK der K and Borchert H-H (1998) On-line coupled HPLC-ESR: Application for separation and characterization of organic radicals. Archiv der Pharmazie } Pharmaceutical and Medicinal Chemistry 331 (2): 40. Osterloh K, Kroll C, MaK der ...
متن کاملSimultaneous determination of aminoglycoside antibiotics in feeds using high performance liquid chromatography with evaporative light scattering detection
A sensitive, rapid and reproducible method has been developed based on high performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) for the simultaneous detection of ten aminoglycoside antibiotics including apramycin, neomycin, amikacin, and gentamicin. The influence of parameters of ELSD on the responses of aminoglycosides was investigated in detail. The targe...
متن کاملHPlC Analysis of Phospholipids by Evaporative laser light-Scattering Detection
oDays High-performance liquid chromatography (HPLC) for analysis of phospholipids has traditionally employed ultraviolet detection of the eluted compounds. The evaporative laser light-scattering detector (ELSD) offers new opportunities for quantitative analysis of lipids. Phospholipids were isolated from crude and degummed oils prepared from soyheans subjected to storage at high moisture conten...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Pharmaceutical and Biomedical Analysis
سال: 2021
ISSN: 0731-7085
DOI: 10.1016/j.jpba.2020.113640